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Methanococcoides vulcani sp. nov., a novel marine methylotrophic methanogen; using betaine, choline and N,N-dimethylethanolamine for methanogenesis, isolated from the Napoli Mud Volcano in the Eastern Mediterranean Sea; and emendation of the genus Methanococcoides

March 17, 2014

A novel strictly anaerobic methylotrophic marine methanogen, strain SLHL33T, was isolated from deep sediment samples covered by an orange microbial mat collected from the Napoli Mud Volcano. Strain SLH33T stained Gram negative, was a non-motile irregular coccus and occurred singly. Cells utilized trimethylamine, dimethylamine, monomethylamine, methanol, betaine, N,N-dimethylethanolamine and choline (N,N,N-trimethylethanolamine) as substrates for growth and methanogenesis. The optimal growth temperature was 30 °C, maximum growth rate was obtained at pH 7.0 with 0.5 M of Na+. Its DNA G+C content was 43.4 mol%. Phylogenetic analyses based on 16S rRNA gene sequences located strain SLH33T within the genus Methanococcoides. The novel isolate was most closely related to M. methylutens (98.8% 16S rRNA gene sequence similarity) but distantly related to M. burtonii (97.6%) and M.alaskense (97.6%). DNA-DNA hybridization studies showed that strain SLH33T was a novel species, DNA-DNA relatedness value below 16% with M. methylutens. We propose the name Methanococcoides vulcani sp. nov., with strain SLH33T (=DSM 26966T, =JCM 19278T) as the type strain.

  •  L’Haridon S,
  •  Chalopin M,
  •  Colombo D,
  •  Toffin L, 2014: Methanococcoides vulcani sp. nov., a novel marine methylotrophic methanogen; using betaine, choline and N,N-dimethylethanolamine for methanogenesis, isolated from the Napoli Mud Volcano in the Eastern Mediterranean Sea; and emendation of the genus Methanococcoides, International Journal of Systematic and Evolutionary Microbiology, doi:10.1099/ijs.0.058289-0. Article (subscription required).
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